在下丘脑实质中，沿着 tanycyte 过程的特殊突起面临着不同的神经和非神经细胞类型。,The Journal of Comparative Neurology

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在下丘脑实质中，沿着 tanycyte 过程的特殊突起面临着不同的神经和非神经细胞类型。
The Journal of Comparative Neurology ( IF 2.5 ) Pub Date : 2020-06-08 , DOI: 10.1002/cne.24965
Roxane Pasquettaz ₁ , Irina Kolotuev ₂ , Antoine Rohrbach ₁ , Cathy Gouelle ₃ , Luc Pellerin _{3,

4,

5} , Fanny Langlet ₁

Affiliation

Tanycytes 是高度特化的室管膜细胞，排列在第三脑室的底部和侧壁。通过它们的细胞体与脑脊液接触，它们将过程发送到弓状核、腹内侧核和下丘脑的背内侧核。在目前的工作中，我们结合转基因和免疫组织化学方法来研究存在于下丘脑实质中，特别是弓状核中的 tanycytes 和神经细胞之间的神经解剖学关联。tdTomato 在 tanycytes 中的特定表达首先允许观察沿 tanycyte 过程及其末端的特殊亚细胞突起，如刺、肿胀、en passant boutons、boutons 或爪子。有趣的是，这些突起接触脑实质中的不同神经细胞，包括血管和神经元，特别是弓状核中的 NPY 和 POMC 神经元。使用荧光和电子显微镜，我们最终观察到这些长链细胞突起包含核糖体、线粒体、不同的囊泡和转运蛋白，表明密集的长链细胞/神经元和长链细胞/血管通信。总而言之，我们的结果为长细胞/神经细胞相互作用奠定了神经解剖学基础，这将有助于进一步了解参与神经内分泌功能调节的细胞间通讯。线粒体、不同的囊泡和转运蛋白，表明密集的 tanycyte/神经元和 tanycyte/血管通信。总而言之，我们的结果为长细胞/神经细胞相互作用奠定了神经解剖学基础，这将有助于进一步了解参与神经内分泌功能调节的细胞间通讯。线粒体、不同的囊泡和转运蛋白，表明密集的 tanycyte/神经元和 tanycyte/血管通信。总而言之，我们的结果为长细胞/神经细胞相互作用奠定了神经解剖学基础，这将有助于进一步了解参与神经内分泌功能调节的细胞间通讯。

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Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma.

Tanycytes are highly specialized ependymal cells that line the bottom and the lateral walls of the third ventricle. In contact with the cerebrospinal fluid through their cell bodies, they send processes into the arcuate nucleus, the ventromedial nucleus, and the dorsomedial nucleus of the hypothalamus. In the present work, we combined transgenic and immunohistochemical approaches to investigate the neuroanatomical associations between tanycytes and neural cells present in the hypothalamic parenchyma, in particular in the arcuate nucleus. The specific expression of tdTomato in tanycytes first allowed the observation of peculiar subcellular protrusions along tanycyte processes and at their endfeet such as spines, swelling, en passant boutons, boutons, or claws. Interestingly, these protrusions contact different neural cells in the brain parenchyma including blood vessels and neurons, and in particular NPY and POMC neurons in the arcuate nucleus. Using both fluorescent and electron microscopy, we finally observed that these tanycyte protrusions contain ribosomes, mitochondria, diverse vesicles, and transporters, suggesting dense tanycyte/neuron and tanycyte/blood vessel communications. Altogether, our results lay the neuroanatomical basis for tanycyte/neural cell interactions, which will be useful to further understand cell‐to‐cell communications involved in the regulation of neuroendocrine functions.

更新日期：2020-06-08

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